Figure 1From: Porcine type I interferons: polymorphic sequences and activity against PRRSVDifferential antiviral activity among polymorphic isoforms of some subtypes of porcine type I IFNs. Antiviral activity against PRRSV and VSV was evaluated as described in Sang et al., 2010 [11], and analyzed based on individual polymorphic isoforms. Authentic expression of individual IFN peptides was confirmed by gel electrophoresis. Peptide concentrations were adjusted to 2 μg/ml to ensure the antiviral differences were not a result of peptide levels in the antiviral assays. Antiviral activity of overexpressed IFN peptides was assayed as the inhibition of virus cytopathic effect, in which 1 unit (U) is defined as the highest dilution that reduced cell loss by 50% according to the Reed-Muench method. Data are means from four duplicates of two independent experiments. *p<0.05, n=4.Back to article page