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Fig. 1 (abstract P-071). | BMC Proceedings

Fig. 1 (abstract P-071).

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Fig. 1 (abstract P-071).

Production of Fab fragment under optimal conditions in a shake-flask culture. Cells at a density of 1 x 106 cells/cm3 were transfected with 5 μg/(106 cells) of DNA (Hc:Lc gene ratio = 3:7) using 10 μg/(106 cells) of PEI in the serum-free medium PSFM-J1.Transfected cells were incubated at 24 °C. a Density of viable cells. Density of untransfected cells (open circles) is also shown. b Concentration of Fab fragments in the culture supernatant. Bars represent the means ± S.D. obtained from eight (a) or three (b) different determinations

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