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Fig. 1 (abstract P-183). | BMC Proceedings

Fig. 1 (abstract P-183).

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Fig. 1 (abstract P-183).

Influence of the capsule material on the expansion of human primary T lymphocytes. a: Polyelectrolyte capsule (SCS/PDAMAC). b: Growth of T lymphocytes encapsulated in SCS/PDADMAC versus alginate/PLL. Growth kinetics of activated peripheral blood T cells, non-encapsulated () and encapsulated in SCS/PDADMAC () or alginate/Poly-L-Lysine (). The cells were cultivated in fresh (solid lines) or conditioned (dotted lines) specialized T cells medium containing phytohemaglutinin (PHA) as activating substance. Starting on day 4, 35% of the growth medium was exchanged every second day. Data represent mean ± SD (n=3). D0: day of encapsulation. Statistical significance indicated by * (p < 0.05). c: Comparison of the relative expansion of T lymphocytes under various culture conditions. Left: IL-2 (rhIL-2, 11 ng mL−1) and PHA (2 μg mL−1) were added either alone or in combination to the cell culture medium of the non-encapsulated cells or co-encapsulated with the cells. No supplements (,), PHA (); IL-2 (,); IL-2/PHA ().QPBL, LG: activation media containing PHA. R10, R0: RPMI1640-based media supplemented or not 10 % FCS. Culture volume was 30 mL (QPBL) or 15 mL (LG, R10 and R0), total capsule volume was always 3 mL. D0: day of encapsulation. Right: Total number of cells produced in non-encapsulated (orange bars) and encapsulated (violet bars) systems and total amounts of additives (rhIL-2, PHA) required. d: Schematic representation of the putative influence of the capsule microenvironment on the T cells signalling after PHA activation. Left: Non-encapsulated T lymphocytes, right: T lymphocytes in SCS/PDADMAC capsules

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