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Fig. 1 (abstract P-317). | BMC Proceedings

Fig. 1 (abstract P-317).

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Fig. 1 (abstract P-317).

In vitro stability and interaction of rhBMP2 with the producer cells. a Stability of recombinant hBMP2: rhBMP2 produced in E.coli (white bars) and in CHO cells (black bars), duration: 120h. Fresh (dotted bars) and end-of-culture (solid bars) ExCell medium. b Binding of hBMP2 to CHO and HEK-293 cells: rhBMP2 produced in E.coli (■,▲) and in CHO cells (). ■ : 3 x 106 CHO cells mL-1 in ProCHO5 medium (solid line). ▲, : 4 x 106 HEK-293 cells mL-1 in ExCell medium (solid lines). rhBMP2 incubated in the respective medium without cells (dashed lines). rhBMP2: 5 ng mL-1, incubation temperature 37°C

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