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Fig. 1 (abstract P-318). | BMC Proceedings

Fig. 1 (abstract P-318).

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Fig. 1 (abstract P-318).

Transfection efficiency and magnetic property of the cells after transfection. a Transfection in serum-free medium at optimal N/P ratios for each polycation. Transfection efficiencies (EGFP+-cells, bars) and viabilities (circle) determined 26h post transfection. Subgroups according to the grafting density/hydrodynamic radii of the corresponding polyplexes: I (violet), II (orange), III (blue). Data represent mean ± S.E.M., n ≥ 3. b Effect of serum on transfection efficiencies of NP@(PDMAEMA528)20 (N/P 20) (violet, dotted bars), NP@(PDMAEMA1037)46 (N/P 15) (orange, stripped bars), and NP@(PDMAEMA1100-b-PDEGMA4350)98 (N/P 20) (green, solid bars). Data represent one experiment carried out in duplicate, with random experimental error shown. c EGFP expression in magnetic and non-magnetic cell fractions after magnetic separation compared to the controls (non-separated cells). Cells were transfected with NP@(PDMAEMA1037)46 (N/P 15), separated 24 h post transfection (t = 0) by magnetically-assisted cell sorting and placed into separated cultures. The bars represent the overall transfection efficiency. Distribution of low (light green), middle (green), and high (dark green) producers within the EGFP-expressing cell fraction. Data represent one experiment carried out in duplicate, with random experimental error shown. d Correlogram between the molecular characteristics of the nano-stars (core diameter, arm density, arm length, number of monomeric units per nano-star), the physicochemical properties of the corresponding polyplexes (hydrodynamic radius, zeta potential), the transfection conditions (N/P ratio, amount of polymer), and the cellular reactions (transfection efficiency, magnetism, viability). Positive correlations are indicated in blue, negative ones in red. Correlation coefficients were not calculated for parameters, which showed no statistically relevant differences (p > 0.01)

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