Fig. 1 (abstract P-323).
a Growth characteristics of CTE-and STE-cultures: comparison of maximum viable cell density achieved when rCHO cells are treated with varying concentrations of tunicamycin. Note, the maximum VCD was obtained on day 6. The % drop in viability at day 6 from time point of inoculation is also plotted. White bars denote VCD of CTE-cultures, Grey bars denote VCD of STE-cultures, White solid circle with dotted lines denote Viability of CTE-cultures, Grey solid circle with dotted lines denote Viability of STE-cultures. b Fold change in CTE IgG titers: fold change of IgG titers in CTE cultures. The IgG titers of day 2 (grey bars) and day 3 (black bars) were normalized w.r.t control day 1 levels. White bars denote day 1 levels. c IgG productivity profile of CTE-cultures: comparison of per-cell productivity in pg/cell-day of CTE-cultures treated with varying concentrations of tunicamycin (30 – 500 ng/mL). White bars denote day 1, grey bars denote day 2 and black bars denote day 3 qP levels. d Fold change in STE IgG titers: fold change of IgG titers in STE cultures. Ratios are calculated w.r.t control day 1 levels. White bars denote day 1, Grey bars denote day 2, Black bars denote day 3 fold-change. e IgG productivity profile of STE-culture: comparison of per-cell productivity in pg/cell-day of STE-cultures when treated with tunicamycin (30 ng/mL – 500 ng/mL). White bars denote qP at day 1, grey bars at day 2 and black bars at day 3. f Productivity profile of Long term adapted cultures: Comparison of productivity profile of control and long term tunicamycin adapted cultures in a batch mode. These long term adapted cells were obtained by culturing CHO cells in presence of 30 ng/mL tunicamycin for over 25 passages. Solid white circle with black line denote control productivity of control cultures and solid black squares with black line denote qP of tunicamycin adapted cells. g Calreticulin/CRT mRNA fold change: comparison of Calreticulin mRNA fold change w.r.t respective control for CTE-and STE-cultures when treated with 30 and 500 ng/mL of tunicamycin.White bars denote CTE-cultures harvested post 24 hrs of tunicamycin treatment. Black bars denote STE-cultures harvested post 24 hrs culturing in fresh media. h UPR mRNA (XBP1s, Grp78, CHOP) fold change: Comparison of XBP1s. Grp78 and CHOP mRNA fold change w.r.t respective control for CTE-and STE-cultures at 30 and 500 ng/mL of tunicamcyin. White solid bars denote XBP1s levels of CTE-cultures, Grey solid bars denote XBP1s levels of STE-cultures, White solid bars with black horizontal lines denote Grp78 levels of CTE-cultures, Grey solid bars with black horizontal lines denote Grp78 levels of STE-cultures, White solid bars with black bubble pattern denote CHOP levels of CTE-cultures, Grey solid bars with black bubble pattern denote CHOP levels of STE-cultures. i IgG Heavy Chain mRNA fold change: comparison of IgG Heavy chain mRNA fold change wrt respective control for CTE-and STE-cultures at 30 and 500 ng/mL. White bars denote CTE-cultures harvested post 24 hrs of tunicamycin treatment. Black bars denote STE-cultures harvested post 24 hrs culturing in fresh media