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Fig. 1 (abstract P-381). | BMC Proceedings

Fig. 1 (abstract P-381).

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Fig. 1 (abstract P-381).

Characterization of spheroid “mini bones”. a Different number (1.000-50.000 cells) of CI-huOB cells were printed into spheroids. b 20.000 CI-huOBs were printed into spheroids and cultivated for indicated time points. c For analyzing spheroid sizes, pictures were taken and quantified by ImageJ. (D/E) 20.000 CI-huOB cells were printed into spheroids and cultivated with (huOB Differentiation Medium) or without a differentiation stimulus for two weeks. Afterwards, bioprinted spheroids were sectioned by a cryo microtome and d stained for Ca2+ deposits (Alizarin Red S) or e stained for alkaline phosphatase activity

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