Table 1 (abstract P-014). Material and methods used for the work described

Cell lines

Recombinant CHO Cells expressing monoclonal antibody

Media

Chemically defined cell culture media

Sub-Culturing

Every 2-3 days, cells are sub-cultured with fresh medium and incubated in CO₂ shaker incubator

Production Run

Production runs were conducted in 1L scale bioreactor in fed batch mode

Analysis

Cell Concentration & Viability (Cedex HiRes),

Product titer using Protein A HPLC, Lactate (YSI)