Table 1 Perfusion Process Details.
From: Novel small scale TFF cell retention device for perfusion cell culture systems
Materials and Equipment | |
|---|---|
Cell Line | rCHOS |
Vessel and Controller | 3L Applikon® Glass Bioreactor Finesse® TruBio™DeltaV |
Additional Equipment | Single-use recirculation Levitronix™ Pump, Single-use Mobius® 20L bags (Harvest and Medium) |
Cell Growth Monitoring - two methods | Vi-CELL® (Trypan blue exclusion method) |
| Â | Biomass by capacitance (iBiomassâ„¢ - Fogaleâ„¢) |
Bioreactor Conditions | |
Vessel Temp (C) | 37.0 |
pH Set Point | 7.0 ( ± 0.2) |
Dissolved Oxygen (DO) | 50% |
Aeration Gas/Location | Air-Oxygen / Open Pipe |
Inoculation Density | 3~5E5 vc/mL |
Inoculation Agitation | 250 rpm (30W/m3) |
Working Volume (L) | 1.5 L |
Daily Cell Bleed | Manually (w/ peristaltic pump) |