Figure 1
From: Model-based strategy for cell culture seed train layout verified at lab scale
(A) simulated courses of Space-Time-Yield (STY) and apparent growth rateover time exemplarily for one scale: a) point in time of minimal STY, b) average value of a) and c) as a cell passaging criterion, c) point in time of maximal STY as a cell passaging criterion, d) average of time at maximal STY and time at 0.9·µmax as a cell passaging criterion. (B) Seed train for CHO-K1 - simulated and experimental courses of viable cell density over time. Passaging of cells at the points in time calculated using average of time at maximal STY and time at 0.9·µmax (criterion d)).The seed train steps were: 1. culture tube (0.0025 L), 2. shake flask (0.070 L), 3: Labfors 5 Cell (2 L)